Monolayer culture of rat parotid acinar cells without basement membrane substrates
نویسندگان
چکیده
منابع مشابه
Rat Parotid Acinar Cells
We used the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6')-carboxyfluorescein to monitor the recovery of the intracellular pH (pH,) of rat parotid acini from an NH+-induced alkaline load. This recovery was markedly inhibited by the loop diuretic bumetanide and by Clremoval, indicating that it is largely due to NH' entry via the basolateral Na'-K+2Clcotransporter. The rate of recov...
متن کاملCalcium metabolism and amylase release in rat parotid acinar cells.
1. A method is described for the isolation of rat parotid acinar cells by controlled digestion of the gland with trypsin followed by collagenase. As judged by Trypan Blue exclusion, electron microscopy, water, electrolyte and ATP concentrations and release of amylase and lactate dehydrogenase, the cells are morphologically and functionally intact. 2. A method was developed for perifusion of aci...
متن کاملCapacitative calcium entry in parotid acinar cells.
The intracellular Ca2+ indicator, fura-2, was used to monitor changes in cytosolic [Ca2+] in parotid acinar cells. When parotid cells were incubated in a medium containing low [Ca2+], and [Ca2+] was restored to the physiological range, there was a small increase in cytosolic [Ca2+]. If, however, the cells were first activated by a muscarinic agonist, and receptor activation was terminated befor...
متن کاملParenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture
Hepatic parenchymal cells from adult rats, established in vitro as a monolayer, have been evaluated by electron microscopy. Within 24 h after the initial seeding, the incubated cells were polygonal and in close apposition with three to six neighboring cells. The ultrastructure of the monolayer cells was examined at this time and after 3 and 10 days of incubation. With the exception of a few enl...
متن کاملNerve-acinar Cell Relationships in the Rat Parotid Gland
Ultrastructural studies have shown that many exocrine glands receive their autonomic innervation through close (180-250 A) apposition of the nerve and acinar cell membranes (1, 5, 14, 16, 17, 20, 23-25). The nerve terminations or varicosities often lie in a groove on the surface of the acinar cell, or are closely apposed to two or more acinar cells by lying in the intercellular space between th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology
سال: 1990
ISSN: 0883-8364,1475-2689
DOI: 10.1007/bf02624613